Description
Aviva Systems Biology H2AA ELISA Kit (Mouse) (OKEH02479) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse H2AA antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated H2AA and incubated. The H2AA found in the sample or standards competes with the biotinylated H2AA for limited binding sites on the immobilized anti-Mouse H2AA antibody. Excess unbound biotinylated H2AA and sample or standard H2AA is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated H2AA captured in the well and inversely proportional to the amount of H2AA which was contained in the sample or standard